… The agar well diffusion method as described by Jorgenson et al. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The authors appreciate the support and assistance of the Ghana National Association of Traditional Healers Ashanti region (GNATH) for their assistance. traditional health practioners, IP. Specialists could administer such medicines as herbal teas, preparations to be chewed and swallowed, poultices, inhaled vapours, or a variety of other applications. Kabo khaji Katha Mimosaceae HD 2 Linn. The activity of the individual isolated compounds was lower than the crude alkaloid fraction, suggesting the possible role of synergism in the activity of the steroidal alkaloids. The vernacular names of plants, uses, methods of preparation, and the administration of remedies were documented. Holarrhena floribunda stem bark extract has strong biofilm formation inhibition properties, which could be … doi: Cox PA.Will tribal knowledge survive the millennium? COVID-19 is an emerging, rapidly evolving situation. The communities are well known for their traditional beliefs and the use of plants for medicine (Ghana Statistical Service, 2014). 1. The significance of medicinal plants as foods INDIGENOUS MEDICINAL PLANTS, TRADITIONAL FOODS, NUTRITION, PREVENT DISEASE Tickets, Fri 27 Nov 2020 at 12:00 | Eventbrite Eventbrite, and certain approved third parties, use functional, analytical and tracking cookies (or similar technologies) to understand your event preferences and provide you with a customised experience. These data can serve as evidential support for the clinical development of a number of medicinal plant remedies as adjuvant therapy. Geneva. Mahmood A, Mahmood A, Malik RN, Shinwari ZK. This could be useful in novel drug discovery and to validate the ethomendicinal knowledge. It is located in the central part of the region (1.15°N and 1.45°N and longitude 6.15°W and 7.00°W) and has an estimated population of about 150,000 with about 72% residing in the rural areas (Ghana Statistical Service, 2014). Seventy medicinal plants used by traditional practitioners for the treatment of skin infections and wounds were documented from the ethnobotanical survey. To this end, the indigenous knowledge on medicinal plants and non-plant remedies has not been systematically documented and studied in the Dawuro … Elikplim Kwesi Ampofo, Isaac Kingsley Amponsah, Evelyn Asante-Kwatia, Francis Ackah Armah, Philip Kobla Atchoglo, Abraham Yeboah Mensah, "Indigenous Medicinal Plants as Biofilm Inhibitors for the Mitigation of Antimicrobial Resistance", Advances in Pharmacological and Pharmaceutical Sciences, vol. Journal of Ethnopharmacology. They were filtered, concentrated on a rotary evaporator, and dried on a water bath. doi: 10.1371/journal.pone.0171896. A total of 85 species belonging to 71 genera and 34 families were documented along with ethnomedicinal uses. MHE showed the highest biofilm formation inhibitory effect with an MBIC of 0.0625 mg/mL (Table 3). A prior informed consent form (translated into the local dialect) was given to the participants to sign after the objectives of the study had been explained. Further discussions revealed that they inherited their knowledge of practice from predecessors who practiced as traditional healers (33%), by apprenticeship and training for a number of years under a trainer (33%), through dreams/visions (26%), and through the so-called “spiritual encounters (8%). Steroidal alkaloids are the major types of phytoconstituents identified in H. floribunda [16,27,28]. Indigenous medicinal plants will be put under the microscope as part of an Australian-first research project to look at commercialisation opportunities for bush medicine. PLoS One. A. Poku, S. Boateng et al., “Integration for coexistence? A. Badmus, O. E. Ekpo, F. Rautenbach, J. L. Marnewick, A. Would you like email updates of new search results? Proportional contributions of plant part…. This work was supported by the Building Stronger Universities (BSU) Grant, WP3. Medicine in your backyard: How Indigenous peoples have used medicinal plants A program available at Wanuskewin Heritage Park shows people plants … Zahoor M, Yousaf Z, Aqsa T, Haroon M, Saleh N, Aftab A, Javed S, Qadeer M, Ramazan H. J Ethnobiol Ethnomed. Lyonia. Epub 2014 Jan 7. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The ethnobotanical survey was carried out in May, 2016, with the help of some members of the Ghana National Association of Traditional Healers, Ashanti region (GNATH), a subunit of the Ghana federation of traditional healers associations (GHAFTRAM). (a), Photomicrographs of adhering bacterial cells (biofilms) stained with 0.1% crystal violet on a glass slide after 24 hours, observed at ×100: (a). The Buchu has been a favourite plant for South African healers for centuries and is inherited from one generation to the next. Extracts that showed antibacterial activity were tested for biofilm inhibitory activity, and the most active plant was subsequently purified to obtain the active constituents. 2013; 148: 714–723. The use of medicinal plants for the treatment of gastrointestinal disorders and ethnodiseases such as diarrhea, stomachache, dysentery, “empacho” (blockage), and bile is a common strategy among indigenous communities. III. Briefly, a sterile glass slide placed in a sterile petri dish was overlaid with 20 mL of Mueller Hinton broth seeded with 100 μL of standardized culture (∼108 CFU/mL). Science. After the incubation, the liquid suspension was removed and the amount of biofilm formed determined as described in Section 2.5.4. The ability of the extracts to considerably inhibit biofilm formation by a strong biofilm producer (i.e., S. aureus) was determined and the minimum biofilm inhibitory concentration (MBIC) recorded. Amjad MS, Zahoor U, Bussmann RW, Altaf M, Gardazi SMH, Abbasi AM. 2020 Oct 27;16(1):65. doi: 10.1186/s13002-020-00417-w. See this image and copyright information in PMC. Seventy plants used by the traditional practitioners for the treatment of skin, wounds, and other infections were documented from the survey (Supplementary Material-Table S1). Briefly, 100 μL of sterile liquid broth medium was inoculated with 10 μL of standardized overnight culture of the selected bacterium, and volumes of plant extracts reconstituted in distilled water were added to obtain concentrations of 10, 5, 2.5, and 1.25 mg/mL. Many Caribbean indigenous plants now identified solely as food crops were also valued by my ancestors for their medicinal properties. No. and Schinz: holarrheline, holadienine, holaromine and holaline,”, K. Appiah, C. Oppong, H. Mardani et al., “Medicinal plants used in the Ejisu-Juaben municipality, Southern Ghana: an ethnobotanical study,”, N. K. Archer, M. J. Mazaitis, J. W. Costerton, J. G. Leid, M. E. Powers, and M. E. Shirtliff, “, A. M. Lannang, S. Anjum, J. G. Tangmouo, K. Krohn, and M. I. Choudhary, “Conessine isolated from. 271. Demographic features of informants; life form, part used, methods of preparation, modes of application and ethnomedicinal uses were documented. The antibiofilm activity of Azadirachta indica extract was employed to mitigate microbiologically influenced corrosion in underground pipe lines as an environmentally benign way of managing corrosion [8]. Bindseil, K. Siems, J. Frevert, and P. M. Abreu, “New trichothecenes isolated from, J. Fotie, D. S. Bohle, M. L. Leimanis, E. Georges, G. Rukunga, and A. E. Nkengfack, “Lupeol long-chain fatty acid esters with antimalarial activity from, D. Kavitha, P. Shilpa, and S. N. Devaraj, “Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica Wall,”, A. Chakraborty and A. H. Brantner, “Antibacterial steroid alkaloids from the stem bark of, B. S. Siddiqui, S. T. Ali, G. H. Rizwani, S. Begum, S. Tauseef, and A. Ahmad, “Antimicrobial activity of the methanolic bark extract of, S. Chusri, P. Na-Phatthalung, T. Siriyong, S. Paosen, and S. P. Voravuthikunchai, “, T. Siriyong, S. Chusri, P. Srimanote, V. Tipmanee, and S. P. Voravuthikunchai, “, T. Siriyong, S. P. Voravuthikunchai, and P. J. Coote, “Steroidal alkaloids and conessine from the medicinal plant, T. Siriyong, P. Srimanote, S. Chusri et al., “Conessine as a novel inhibitor of multidrug efflux pump systems in, A. Reza, J. M. Sutton, and K. M. Rahman, “Effectiveness of efflux pump inhibitors as biofilm disruptors and resistance breakers in gram-negative (ESKAPEE) bacteria,”, Z. Li-Na, G. Xiao-Lei, D. Ting-Ting, G. Hui-Yuan, and S. Bo-Hang, “Antibacterial steroidal alkaloids from, A. Tanwar, R. Chawla, A. S. Chakotiya et al., “Effect of. Three extracts, pet-ether (PHE), ethyl acetate (EHE), and methanol (MHE) fractions, were prepared from the crude extract and tested for biofilm inhibitory activity. They were then stored in a cool dry place until required for use. Adhikari JN, Bhattarai BP, Rokaya MB, Thapa TB. This is consistent with a previous study by Li-Na et al. Ethno-medicinal uses of vertebrates in the Chitwan-Annapurna Landscape, central Nepal. Antimicrobial resistance (AMR) has become a major public health concern worldwide as it reduces the effectiveness of antibiotic therapies and increases morbidity, mortality, and health care costs [1]. After 24 and 48 hours of incubation, the slides were washed with normal saline to remove the floating planktonic bacteria, and the biofilm was fixed by heating the slide. 2021 Mar 1;267:113200. doi: 10.1016/j.jep.2020.113200. Ethical approval for the study was obtained from the chiefs of the studied areas and the GNATH. In the past two decades, the links between bio-cultural diversity and the richness of plant species in South Africa have contributed to ongoing attempts to develop traditional medicines for use in the modern complementary and alternative medicines sector. indigenous medicinal plants knowledge from their family members and friends secretly. At a concentration of 1.25 mg/mL, MHF reduced S. aureus from a high biofilm producer (OD > 1.38) to a low biofilm-producing organism with an OD < 0.24 (Figure 4(b)). The wells were then washed with sterile water and dried. The comparative assessment with reported literature revealed 15% resemblance and 6% variation to previous data;however79% uses of the reported species were recorded for the first time. G. vignei, methanol extract (MGV) on the other hand, reduced the biofilm production of S. aureus to a moderate biofilm producer at concentrations of 5 to 10 mg/mL (Figure 4(b)). After incubation, the planktonic cells were aspirated and the wells were washed with sterile water to remove the free-floating bacteria. Plants synthesise hundreds of chemical compounds for functions including defence against insects, fungi, diseases, and herbivorous mammals.. Fig 1. MEDICINAL AND CULTURAL USE OF INDIGENOUS PLANTS Indigenous medicinal plants are used by more than 60% of South Africans in their health care needs or cultural practices (Table 1). Wood Science and Forest Products Laboratory, Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli-791 We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. They comprised 43% men and 57% women aged between 21 and 80 years. We are committed to sharing findings related to COVID-19 as quickly as possible. (a-b) Graphs showing the effect of different concentrations of extracts on the amount of biofilm formed (optical density-OD) by. Demographic data of local informants (THPs. Bioassay-guided fractionation of an alkaloidal extract prepared from the methanol fraction led to the isolation of three steroidal alkaloids, namely, holonamine, holadienine, and conessine. For a long time, the people of Saudi Arabia have been using medicinal plants (MPs) as conventional medicine to heal diverse human and livestock diseases. Bacterial biofilms stained with 0.1 % crystal violet ( CV ) assay as described in Section 2.5.4 ; life,... Gastrointestinal diseases among the tested plants, uses, methods of preparation, methanol. Of pet-ether, EtOAc, and herbivorous mammals J Ethnopharmacol with efflux pump may... Survey and personal visits were made to their homes and practice sites makes the current study reveals that of! 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